DPD spectrophotometry bụ usoro ọkọlọtọ maka ịchọpụta chlorine fọdụrụnụ n'efu na mkpokọta chlorine fọdụrụnụ n'ụkpụrụ mba China “Mkpụrụokwu dị mma na usoro nyocha” GB11898-89, nke American Public Health Association, American Water Works Association na njikwa mmetọ mmiri mebere. Federation. N'ime "Ụzọ Ule Ọkọlọtọ maka Mmiri na Mmiri", emepụtara usoro DPD kemgbe mbipụta nke iri na ise ma na-atụ aro ka usoro ọkọlọtọ maka ịnwale chlorine dioxide.
Uru nke usoro DPD
Ọ nwere ike ikewapụ chlorine dioxide n'ụdị chlorine dị iche iche (gụnyere chlorine fọdụrụnụ n'efu, chlorine residual na chlorite, wdg), na-eme ka ọ dịkwuo mfe ịme ule colorimetric. Usoro a ezighi ezi dị ka titration amperometric, mana nsonaazụ ya zuru oke maka ebumnuche izugbe.
ụkpụrụ
N'okpuru ọnọdụ nke pH 6.2 ~ 6.5, ClO2 na-emeghachi omume na DPD mbụ iji mepụta ogige uhie, ma ego ahụ na-egosi na ọ bụ nanị otu ụzọ n'ụzọ ise nke mkpokọta chlorine dị (nke kwekọrọ na mbenata ClO2 na ions chlorite). Ọ bụrụ na ihe nlele mmiri na-eme acidified n'ihu iodide, chlorite na chlorate na-emeghachikwa omume, na mgbe a na-ewepụ ya site na mgbakwunye nke bicarbonate, agba nke na-esi na ya kwekọrọ na mkpokọta chlorine dị na ClO2. Enwere ike igbochi nnyonye anya nke chlorine n'efu site na ịgbakwunye glycine. Ihe ndabere bụ na glycine nwere ike gbanwee chlorine efu ozugbo ka ọ bụrụ chlorinated aminoacetic acid, mana enweghị mmetụta na ClO2.
Potassium iodate ọkọlọtọ ngwaahịa ngwọta, 1.006g/L: tụọ 1.003g potassium iodate (KIO3, Fikiere na 120 ~ 140 ° C maka 2 awa), igbari na elu-ọcha mmiri, na nyefee 1000ml olu.
Ghichaa iko a na-atụ ihe na akara ma gwakọta ya.
Potassium iodate ọkọlọtọ ngwọta, 10.06mg/L: Were 10.0ml nke ngwaahịa ngwọta (4.1) na a 1000ml volumetric flask, tinye banyere 1g potassium iodide (4.5), tinye mmiri itughari na akara, na mix. Kwadebe ụbọchị eji na karama aja aja. 1.00ml nke ọkọlọtọ ọkọlọtọ nwere 10.06μg KIO3, nke bụ 1.00mg/L dị chlorine.
Ihe nchekwa phosphate: gbazee 24g disodium anhydrous disodium hydrogen phosphate na 46g potassium dihydrogen phosphate anhydrous na mmiri distilled, wee gwakọta n'ime 100ml nke mmiri distilled na 800mg EDTA disodium nnu gbazere. Jiri mmiri distilled gbanye na 1L, tinyezie 20mg mercuric chloride ma ọ bụ 2 tụlee nke toluene iji gbochie uto ebu. Ịgbakwunye 20 mg nke mercuric chloride nwere ike iwepụ nnyonye anya nke ọnụọgụ iodide nke nwere ike ịdịgide mgbe ị na-atụ chlorine efu. (Rịba ama: Mercury chloride na-egbu egbu, jiri nlezianya mee ihe ma zere iri nri)
N, N-diethyl-p-phenylenediamine (DPD) egosi: igbari 1.5g DPD sulfate pentahydrate ma ọ bụ 1.1g anhydrous DPD sulfate na chlorine-free distilled mmiri nwere 8ml1 + 3 sulfuric acid na 200mg EDTA disodium nnu, dilute na 1 liter. na karama iko n'ala aja aja, na-echekwa n'ebe gbara ọchịchịrị. Mgbe ihe ngosi na-ada ada, ọ dị mkpa ka emegharịa ya. Lelee uru nnabata nke ihe nlele oghere,
Ọ bụrụ na uru nnabata nke oghere na 515nm gafere 0.002/cm, ọ dị mkpa ka ahapụ ya nrụgharị ahụ.
Potassium iodide (KI kristal)
Sodium arsenite ngwọta: igbari 5.0g NaAsO2 na distilled mmiri na itughari ka 1 liter. Mara: NaAsO2 na-egbu egbu, zere iri nri!
Ihe ngwọta nke Thioacetamide: gbazee 125 mg nke thioacetamide na 100 ml nke mmiri a na-ekpo ọkụ.
Ngwọta Glycine: gbazee 20g glycine na mmiri na-enweghị chlorine wee gbasaa ruo 100ml. Ụlọ ahịa oyi kpọnwụrụ. Ekwesịrị ịmegharị ya mgbe turbidity pụtara.
Sulfuric acid ngwọta (ihe dị ka 1mol/L): Gbarie 5.4ml lekwasịrị H2SO4 n'ime 100ml distilled mmiri.
Sodium hydroxide ngwọta (ihe dị ka 2mol/L): tụọ 8g NaOH wee gbarie ya na 100ml mmiri dị ọcha.
Usoro nhazi (na-arụ ọrụ).
Na usoro nke 50 colorimetric tubes, tinye 0.0, 0.25, 0.50, 1.50, 2.50, 3.75, 5.00, 10.00ml nke potassium iodate ọkọlọtọ ngwọta, n'otu n'otu, tinye banyere 1g nke potassium iodide na 0.5ml nke sulfuric acid ngwọta. guzo maka nkeji 2, wee tinye 0.5ml sodium hydroxide ngwọta na itughari na akara. Ngwunye dị na karama ọ bụla bụ otu 0.00, 0.05, 0.10, 0.30, 0.50, 0.75, 1.00, na 2.00 mg/L nke chlorine dị. Tinye 2.5ml nke phosphate buffer na 2.5ml nke ihe ngosi DPD, gwakọta nke ọma, ozugbo (n'ime nkeji 2) tụọ nnabata na 515nm site na iji cuvette 1-inch. See usoro mgbanaka wee chọta nhata nkwụghachi azụ.
Nzọụkwụ mkpebi
Chlorine dioxide: Tinye 1ml nke glycine ngwọta na 50ml nke mmiri sample na mix, wee tinye 2.5ml nke phosphate buffer na 2.5ml nke DPD egosi ngwọta, mix ọma, na-atụle absorbance ozugbo (n'ime 2 nkeji) (agụ bụ G).
Chlorine dioxide na chlorine dị n'efu: Were ihe nlele mmiri 50ml ọzọ, tinye 2.5ml phosphate buffer na 2.5ml DPD egosi ngwọta, gwakọta nke ọma, ma tụọ nnabata ahụ ozugbo (n'ime nkeji 2) (agụmakwụkwọ bụ A).
7.3 Chlorine dioxide, chlorine free dị na chlorine jikọtara ọnụ: Were 50ml ọzọ nke mmiri nlele, gbakwunye ihe dị ka 1g nke potassium iodide, tinye 2.5ml nke phosphate buffer na 2.5ml nke ihe ngosi DPD, gwakọta nke ọma, wee tụọ nnabata ahụ ozugbo (n'ime ya). Nkeji 2) (Ọgụgụ bụ C).
Mkpokọta chlorine dịnụ gụnyere chlorine dioxide efu, chlorite, chlorine residual free na chlorine fọdụrụnụ jikọtara: Mgbe ị nwetachara ịgụ C, tinye 0.5ml sulfuric acid ngwọta na mmiri mmiri n'otu karama colorimetric, na mix Mgbe i guzoro ọtọ maka nkeji abụọ, gbakwunye 0,5 ml sodium hydroxide ngwọta, gwakọta ma tụọ nnabata ahụ ozugbo (ọgụgụ bụ D).
ClO2=1.9G (gbakọrọ ka ClO2)
Chlorine dị n'efu=AG
Ngwakọta chlorine dị = CA
Mgbakọta chlorine =D
Chlorite=D-(C+4G)
Mmetụta Manganese: Ihe kacha mkpa na-etinye aka na mmiri ọṅụṅụ bụ manganese oxide. Mgbe ịgbakwunye phosphate buffer (4.3), tinye 0.5 ~ 1.0ml sodium arsenite ngwọta (4.6), wee tinye DPD egosi iji tụọ absorbance. Wepụ ọgụgụ a n'ọgụgụ A iji kpochapụ
Wepu nnyonye anya na manganese oxide.
Mmetụta okpomọkụ: N'ime usoro nyocha niile dị ugbu a nke nwere ike ịmata ọdịiche dị na ClO2, chlorine free na chlorine jikọtara ọnụ, gụnyere amperometric titration, usoro iodometric na-aga n'ihu, wdg, okpomọkụ ga-emetụta izi ezi nke ọdịiche ahụ. Mgbe okpomọkụ dị elu, a ga-akpalite chlorine (chloramine) jikọtara ọnụ na mmeghachi omume n'ọdịnihu, na-ebute nsonaazụ dị elu nke ClO2, karịsịa chlorine efu. Ụzọ mbụ nke njikwa bụ ịchịkwa okpomọkụ. N'ihe dị ka 20 Celsius C, ị nwekwara ike ịgbakwunye DPD na nlele mmiri wee gwakọta ya, wee tinye 0.5ml thioacetamide ngwọta (4.7) ozugbo iji kwụsị chlorine residual (chloramine) sitere na DPD. Mmeghachi omume.
Mmetụta nke oge colorimetric: N'otu aka ahụ, agba uhie nke ClO2 na DPD na-egosi adịghị eguzosi ike. Ka agba gbara ọchịchịrị, ka ọ na-ada ngwa ngwa. N'aka nke ọzọ, dị ka phosphate buffer ngwọta na DPD egosi na-agwakọta ka oge na-aga, ha onwe ha ga-agwụkwa. Na-emepụta agba uhie ụgha, na ahụmahụ egosila na oge a na-adabere na agba agba bụ isi ihe na-akpata mbelata nkenke data. Ya mere, ime ka usoro ọ bụla na-arụ ọrụ ọsọ ọsọ ka ị na-achịkwa nhazi nke oge ejiri na nzọụkwụ ọ bụla dị oké mkpa iji melite nkenke. Dabere na ahụmịhe: mmepe agba na ntinye n'okpuru 0.5 mg / L nwere ike kwụsie ike maka ihe dị ka nkeji 10 ruo 20, mmepe agba na mkpokọta ihe dị ka 2.0 mg / L nwere ike kwụsie ike maka ihe dịka 3 ruo 5 nkeji, yana mmepe agba na ntinye n'elu 5.0 mg / L ga-akwụsi ike maka ihe na-erughị 1 nkeji.
NkeLH-P3CLOnke Lianhua na-enye ugbu a bụ obere ihemita chlorine fọdụrụnụnke na-akwado usoro fotometric DPD.
Onye nyocha ahụ edobela ogologo wavelength na mkpụkọ. Naanị ị ga-agbakwunye reagents na ịrụ colorimetry iji nweta nsonaazụ chlorine fọdụrụ ngwa ngwa, mkpokọta chlorine fọdụrụnụ na chlorine dioxide n'ime mmiri. Ọ na-akwado ọkụ batrị na ọkụ ime ụlọ, na-eme ka ọ dị mfe iji ma n'èzí ma ọ bụ na ụlọ nyocha.
Oge nzipu: Mee-24-2024
